CURRENT UNDERSTANDING OF ANTI-HUMAN GLOBULIN REAGENTS – A REVIEW

Abstract

Anti – human globulin (AHG) reagents are commonly used in compatibility tests prior to transfusion. Cold agglutinins 
or irregular antibodies usually affect the accuracy in functioning of anti – human globulin reagents as they are associated 
with complement system. Though various applications and types of anti – human globulin reagents have been known, 
still there is need for clarification of influencing factors, characteristics and adequate selection prior to transfusion. In 
this review, basic study methods and practices in clinical settings are discussed including anti – human globulin reagents, 
advantages and disadvantages of various reagents in presence of complement – fixing antibodies and cold agglutinins, 
exploration of adequate mechanisms through which anti – human globulin reagents detection is influenced by 
complement system, and describe the adequate and suitable selection of anti – human globulin for the detection of 
antibodies which are significant clinically.